Insertional gene fusion technology.

N Doi; H Yanagawa

doi:10.1016/s0014-5793(99)00991-6

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PubMed · 10486551

Insertional gene fusion technology.

N Doi·H Yanagawa

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Abstract

The classical 'end to end' gene fusion technique has widely been used for monitoring gene expression, biological screening and purification of recombinant proteins. Recent progress with the 'insertional' gene fusion approach, on the other hand, has demonstrated that this technique can be utilized for membrane protein topology analysis, display of randomized protein libraries and design of biosensor proteins. In this review, we describe examples of insertional gene fusion and compare the old and new gene fusion techniques.

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Artificial Gene Fusion Bacterial Proteins Escherichia coli Green Fluorescent Proteins Humans Luminescent Proteins Membrane Proteins Models, Biological Mutagenesis, Insertional Peptide Library Recombinant Fusion Proteins Journal Article Review

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N Doi, H Yanagawa. 1999-08-20. Insertional gene fusion technology.. https://doi.org/10.1016/s0014-5793(99)00991-6

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