Chip and apterous physically interact to form a functional complex during Drosophila development.

D J van Meyel; D D O'Keefe; L W Jurata; S Thor; G N Gill; J B Thomas

doi:10.1016/s1097-2765(00)80373-1

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Chip and apterous physically interact to form a functional complex during Drosophila development.

D J van Meyel·D D O'Keefe·L W Jurata·S Thor·G N Gill·J B Thomas

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Abstract

LIM homeodomain (LIM-HD) proteins play key roles in a variety of developmental processes throughout the animal kingdom. Here we show that the LIM-binding protein Chip acts as a cofactor for the Drosophila LIM-HD family member Apterous (Ap) in wing development. We define the domains of Chip required for LIM-HD binding and for homodimerization and show that mutant proteins deleted for these domains act in a dominant-negative fashion to disrupt Ap function. Our results support a model for multimeric complexes containing Chip and Ap in transcriptional regulation. This model is confirmed by the activity of a chimeric fusion between Chip and Ap that reconstitutes the complex and rescues the ap mutant phenotype.

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Animals Animals, Genetically Modified Dimerization Drosophila Drosophila Proteins Gene Expression Regulation, Developmental Genes, Reporter Homeodomain Proteins Insect Proteins LIM-Homeodomain Proteins Nuclear Proteins Recombinant Proteins Transcription Factors Wings, Animal Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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D J van Meyel, D D O'Keefe, L W Jurata, S Thor, G N Gill, J B Thomas. 1999. Chip and apterous physically interact to form a functional complex during Drosophila development.. https://doi.org/10.1016/s1097-2765(00)80373-1

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