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Hypoxia-ischemia-induced apoptotic cell death correlates with IGF-I mRNA decrease in neonatal rat brain.

T F Clawson·S J Vannucci·G M Wang·L B Seaman·X L Yang·W H Lee
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Abstract

Hypoxia-ischemia induces apoptotic and necrotic cell death, which results partially from persistent alterations in cellular energy homeostasis. Insulin-like growth factor I (IGF-I) is an anabolic pleiotrophic factor required by developing neurons for their optimal proliferation, differentiation, and survival. To determine how cell death and changes in IGF-I gene expression relate to the extent of hypoxia-ischemia, we evaluated the time course of apoptosis in a neonatal hypoxia-ischemia model in relation to the cellular distribution of IGF-I and IGFBP5 mRNA. Severe hypoxia-ischemia results in an immediate decrease in neuronal IGF-I and IGFBP5 mRNA. The decrease in neuronal IGF-I mRNA was concurrent with an increase in the number of apoptotic cells. It is conceivable that the immediate decrease in IGF-I gene expression may contribute to the impending neuronal death and selective vulnerability of myelinogenesis during the perinatal period.

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AnimalsAnimals, NewbornApoptosisBrainBrain IschemiaCell DeathHypoxiaInsulin-Like Growth Factor Binding Protein 5Insulin-Like Growth Factor IRNA, MessengerRatsRats, WistarComparative StudyJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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T F Clawson, S J Vannucci, G M Wang, L B Seaman, X L Yang, W H Lee. Hypoxia-ischemia-induced apoptotic cell death correlates with IGF-I mRNA decrease in neonatal rat brain.. https://doi.org/10.1159/000014599

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