Differences in K-ras codon 12 mutation frequency between "high-risk" and "low-risk" HPV-infected samples.

C D Golijow; S A Mourón; M A Gómez; F N Dulout

doi:10.1006/gyno.1999.5528

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PubMed · 10502435

Differences in K-ras codon 12 mutation frequency between "high-risk" and "low-risk" HPV-infected samples.

C D Golijow·S A Mourón·M A Gómez·F N Dulout

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Abstract

OBJECTIVES: The current study was designed to determine the association between K-ras point mutations and the presence of high- and low-risk HPV types in noncancerous samples. METHODS: One hundred fifty-five noncancerous cytological samples from genital specimens positive for HPV-6, -16, or -18 were analyzed for codon 12 ras point mutations. DNA samples were subjected to nested PCR for the detection of HPV genome. Mutations at the first and second bases of K-ras codon 12 were detected using specific enriched PCR. RESULTS: Eleven percent of the HPV-positive samples analyzed showed mutation in K-ras codon 12 (17 of 155). Mutations were detected in 8 of 53 HPV-16- (15%) and 8 of 38 HPV-18-positive samples (21%). Nevertheless, when HPV-6-positive samples were screened for K-ras mutations only 1 of 64 samples was found mutated (1.56%). Statistical analysis using the chi(2) test showed a highly significant difference (chi(2) = 9.865, P < 0.01) when the rate of mutation for samples positive for high-risk HPV types (16 and 18) was compared with the frequency found in low-risk-infected samples (HPV-6). CONCLUSION: These results could be considered an indicator of the association between K-ras codon 12 mutations and the presence of high-risk HPV types.

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DNA, Viral Female Genes, ras Humans Mutation Papillomaviridae Papillomavirus Infections Risk Factors Tumor Virus Infections Uterine Cervical Neoplasms Comparative Study Journal Article

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C D Golijow, S A Mourón, M A Gómez, F N Dulout. 1999. Differences in K-ras codon 12 mutation frequency between "high-risk" and "low-risk" HPV-infected samples.. https://doi.org/10.1006/gyno.1999.5528

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