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A generic protein purification method for protein complex characterization and proteome exploration.

G Rigaut·A Shevchenko·B Rutz·M Wilm·M Mann·B Séraphin
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Abstract

We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity, or function. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms.

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Affinity LabelsAmino Acid SequenceElectrophoresis, Polyacrylamide GelMethodsMolecular Sequence DataProteinsProteomeJournal ArticleResearch Support, Non-U.S. Gov't

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G Rigaut, A Shevchenko, B Rutz, M Wilm, M Mann, B Séraphin. 1999. A generic protein purification method for protein complex characterization and proteome exploration.. https://doi.org/10.1038/13732

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