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PubMed · 10511631

Cochlear gene transfer: round window versus cochleostomy inoculation.

T Stöver·M Yagi·Y Raphael
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Abstract

Two possible approaches for cochlear gene transfer have been inoculation via the round window membrane and through a cochleostomy. The aim of this study was to determine which of the two is more effective. Using both approaches, normal-hearing and deafened guinea pigs were inoculated with adenovirus carrying the reporter gene lacZ. After 5 days, the animals were killed and the cochlear tissue was stained with X-gal. The distribution and intensity of staining was estimated by a score system developed to compare gene transfer results between animals. We found that gene transfer via the cochleostomy resulted in a better distribution throughout the cochlea and in higher staining intensity, due to more efficient transfection. Auditory brainstem response (ABR) results showed that neither virus inoculation through a cochleostomy nor through the round window membrane had a significant effect on the click-ABR threshold measured on day 5 following virus injection. Gene transfer via both approaches was also found to be more effective in deafened animals than in hearing animals.

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AdenoviridaeAnimalsCochleaDeafnessEvoked Potentials, Auditory, Brain StemGene Transfer TechniquesGenetic TechniquesGenetic VectorsGuinea PigsInjectionsReference ValuesRound Window, EarStaining and LabelingTransfectionJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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T Stöver, M Yagi, Y Raphael. 1999. Cochlear gene transfer: round window versus cochleostomy inoculation.. https://doi.org/10.1016/s0378-5955(99)00115-x

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