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A unique bFGF-responsive transcriptional element.

M A Mohideen·A Hruska-Hageman·M Nilsen-Hamilton
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Abstract

The mitogen-regulated protein/proliferin (mrp/plf) genes encode closely related proteins that stimulate cell proliferation and angiogenesis. Basic fibroblast growth factor (bFGF) increases mrp/plf mRNA and protein production by 3T3 cells. Although the three cloned mrp/plf gene promoters are over 97% identical, only mrp3 is transcriptionally activated by bFGF. A series of truncated mrp3 promoter sequences were tested to determine the minimal promoter sequence necessary for bFGF-responsive transcription. Within the minimal bFGF-responsive mrp3 promoter fragment, a putative FGF-regulatory element (FRE) was identified. Nuclear factors that bind the FRE are present in 3T3 cells. When present upstream of a thymidine kinase basal promoter, the FRE exhibits high transcriptional activity and responds to bFGF. Thus, the FRE is a strong transcriptional element that is regulated by bFGF and that may participate in regulating the mrp3 gene and perhaps other FGF-regulated genes.

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3T3 CellsAnimalsBase SequenceDihydrolipoyllysine-Residue AcetyltransferaseFibroblast Growth Factor 2Fungal ProteinsGelatinasesGene Expression RegulationGlycoproteinsIntercellular Signaling Peptides and ProteinsMatrix Metalloproteinase 3MiceMolecular Sequence DataProlactinPromoter Regions, GeneticProto-Oncogene Proteins c-fosProto-Oncogene Proteins c-junPyruvate Dehydrogenase ComplexRegulatory Sequences, Nucleic AcidResponse ElementsRibosomal ProteinsSequence Homology, Nucleic AcidTetradecanoylphorbol AcetateThymidine KinaseTranscription, GeneticComparative StudyJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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M A Mohideen, A Hruska-Hageman, M Nilsen-Hamilton. 1999-09-03. A unique bFGF-responsive transcriptional element.. https://doi.org/10.1016/s0378-1119(99)00306-6

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