Micro-perfusion flow cell for imaging cultured cells.

N Agnihotri; W S Kisaalita; C H Keith

doi:10.2144/99274st01

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Micro-perfusion flow cell for imaging cultured cells.

N Agnihotri·W S Kisaalita·C H Keith

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Abstract

We present a unique design for a flow cell with a small working volume that allows rapid displacement of media viewed under high power and short working distance objectives. The flow cell has a small internal depth (ca. 0.033 cm) and volume (ca. 0.05 mL) and is easy to handle. Made of Delrin, the flow cell is biologically inert. We have used the flow cell for fluorescence imaging of PC12 cells loaded with tetramethylrhodamine dextran (TMRD) and other dyes.

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Animals Cell Culture Techniques Cell Physiological Phenomena Cyclic AMP Cytological Techniques Fluorescein Fluorescent Dyes Humans Mice Microscopy, Fluorescence PC12 Cells Perfusion Rats Rhodamines Tumor Cells, Cultured Research Support, Non-U.S. Gov't Technical Report Journal Article

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N Agnihotri, W S Kisaalita, C H Keith. 1999. Micro-perfusion flow cell for imaging cultured cells.. https://doi.org/10.2144/99274st01

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