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MAD data collection - current trends.

M A Walsh·G Evans·R Sanishvili·I Dementieva·A Joachimiak
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Abstract

The multiwavelength anomalous dispersion (MAD) method of protein structure determination is becoming a routine technique in protein crystallography. The increased number of wavelength-tuneable synchrotron beamlines capable of performing challenging MAD experiments, coupled with the widespread availability of charge-coupled device (CCD) based X-ray detectors with fast read-out times have brought MAD structure determination to a new exciting level. Ultrafast MAD data collection is now possible and, with the widespread use of selenium in the form of selenomethionine for phase determination, the method is growing in popularity. Recent developments in crystallographic software are complementing the above advances, paving the way for rapid protein structure determination. An overview of a typical MAD experiment is described, with emphasis on the rates and quality of data acquisition now achievable at third-generation synchrotron sources.

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ChaperoninsCrystallography, X-RayFreezingProteinsSelenomethionineSoftwareSynchrotronsJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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M A Walsh, G Evans, R Sanishvili, I Dementieva, A Joachimiak. 1999. MAD data collection - current trends.. https://doi.org/10.1107/s0907444999008392

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