Normalization of array hybridization experiments in differential gene expression analysis.

B Eickhoff; B Korn; M Schick; A Poustka; J van der Bosch

doi:10.1093/nar/27.22.e33

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Normalization of array hybridization experiments in differential gene expression analysis.

B Eickhoff·B Korn·M Schick·A Poustka·J van der Bosch

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Abstract

For detecting and confirming differentially expressed genes it is necessary to have a trustworthy reference. So called 'housekeeping genes' are frequently used for this purpose as internal standard. However, if the influence of new experimental conditions is to be analyzed it is not safe to assume a priori that the expression of these genes is not affected. Therefore two synthetic poly(A)-RNAs were generated by PCR and in vitro transcription. They were used as external standards for normalization of northern blots and cDNA arrays where non-regulated genes as internal reference were not available.

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Ampicillin Resistance Blotting, Northern DNA, Complementary Gene Expression Profiling Humans Kanamycin Resistance Nucleic Acid Hybridization Polymerase Chain Reaction RNA Reference Standards Tumor Cells, Cultured Journal Article

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B Eickhoff, B Korn, M Schick, A Poustka, J van der Bosch. 1999-11-15. Normalization of array hybridization experiments in differential gene expression analysis.. https://doi.org/10.1093/nar%2F27.22.e33

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