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A single immunization with a plasmid encoding hepatitis C virus (HCV) structural proteins under the elongation factor 1-alpha promoter elicits HCV-specific cytotoxic T-lymphocytes (CTL).

Y Nishimura·A Kamei·S Uno-Furuta·S Tamaki·G Kim·Y Adachi·K Kuribayashi·Y Matsuura·T Miyamura·Y Yasutomi
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Abstract

Recent studies have raised the possibility that DNA-based vaccination may prove useful for generating virus-specific cytotoxic T-lymphocytes (CTL) responses. Recently, a plasmid containing the human elongation factor 1alpha(EF1-alpha) promoter, pEF321, was reported to be a versatile expression vector for gene expression in mammalian cells in vitro. In the present study, we assessed the capability of a novel plasmid, pEFCE1E2, encoding hepatitis C virus (HCV) structural proteins (core, E1 and E2) under the EF1-alpha promoter to generate CTL against HCV in vivo. BALB/c mice were immunized with the pEFCE1E2 but not with a plasmid possessing the same cDNA under the cytomegalovirus developed HCV-specific effector cells by a single immunization. These effector cells elicited by pEFCE1E2 immunization were CD8(+) and major histocompatibility complex class I restricted. These studies provided evidence for the potential utility of the EF1-alpha promoter for development of DNA vaccines against HCV infections.

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Amino Acid SequenceAnimalsCytomegalovirusCytotoxicity, ImmunologicDNA, ViralHepacivirusHumansLymphocyte ActivationMiceMice, Inbred BALB CMolecular Sequence DataPeptide Elongation Factor 1PlasmidsPromoter Regions, GeneticT-Lymphocytes, CytotoxicVaccines, DNAViral Hepatitis VaccinesViral Structural ProteinsComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Y Nishimura, A Kamei, S Uno-Furuta, S Tamaki, G Kim, Y Adachi, K Kuribayashi, Y Matsuura, T Miyamura, Y Yasutomi. 1999-11-12. A single immunization with a plasmid encoding hepatitis C virus (HCV) structural proteins under the elongation factor 1-alpha promoter elicits HCV-specific cytotoxic T-lymphocytes (CTL).. https://doi.org/10.1016/s0264-410x(99)00270-4

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