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PubMed · 1054824

A new complement function: solubilization of antigen-antibody aggregates.

G W Miller·V Nussenzweig
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Abstract

Antigen-antibody aggregates are solubilized when incubated with fresh serum at 37 degrees, yielding immune-complexes of relatively small molecular weight which contain antigen, antibody, and complement (C3)determinants. Solubilization is complement-dependent,requires free Mg++ but not Ca++, and proceeds in sera from C4- or C5-deficient animals. It is accelerated in the presence of Ca++ in normal or C4-deficient guinea pig serum, suggesting involvement of the Cl-bypass activation of the properdin system. Immune precipitates can also be solubilized by monovalent fragments (Fab) of antibodies directed against determinants of the antibody molecules included in the antigen-antibody lattice. Similarly, it is suggested that complement-mediated solubilization might be induced by the combination of a complement fragment with the antibody in the immune-aggregate.

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AnimalsAntibodiesAntigen-Antibody ComplexCalciumCattleComplement System ProteinsImmunoglobulin Fab FragmentsImmunoglobulin Fc FragmentsImmunoglobulin GMagnesiumMiceMice, Inbred CBARabbitsSerum Albumin, BovineSerum Albumin, Radio-IodinatedSolubilityJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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G W Miller, V Nussenzweig. 1975. A new complement function: solubilization of antigen-antibody aggregates.. https://doi.org/10.1073/pnas.72.2.418

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