PubMed · 10549528
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P J Webster, P M Macdonald. 1999. Screening expression libraries with solution-based assay.. https://doi.org/10.1385/1-59259-676-2%3A257
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Recent studies have generated interest in the use of the homologous recombination system of bacteriophage lambda for genetic engineering. The system, called Red, consists primarily of three proteins: lambda exonuclease, which processively digests the 5'-ended strand of a dsDNA end; beta protein, which binds to ssDNA and promotes strand annealing; and gamma protein, which binds to the bacterial RecBCD enzyme and inhibits its activities. These proteins induce a 'hyper-rec' state in Escherichia coli and other bacteria, in which recombination events between DNA species with as little as 40 bp of shared sequence occur at high frequency. Red-mediated recombination in the hyper-rec bacterium proceeds via a number of different pathways, and with the involvement of different sets of bacterial proteins, depending in part on the nature of the recombining DNA species. The role of high-frequency double-strand break repair/recombination in the life cycle of the lambdoid phages is discussed.
We present a model to describe electrical conductivity along the DNA double helix. In this model, DNA is considered as a one-dimensional disordered system, and electrons are transported via variable range hopping between localized states. Thermal structural fluctuations in DNA further localize electronic wave functions, giving rise to a temperature-dependent localization length. The model quantitatively explains the temperature dependence of the conductivity observed in the lambda phage DNA (lambda-DNA).
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