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HLA-A typing by reference strand-mediated conformation analysis (RSCA) using a capillary-based semi-automated genetic analyser.

D M Turner·A Poles·J Brown·J R Argüello·J A Madrigal·C V Navarrete
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Abstract

HLA typing of class I loci by reference strand-mediated conformation analysis (RSCA) using a slab gel genetic analyser has been described. This study adapted the method for use in the capillary based ABI PRISM 310. Control DNA samples were used to create a database of mobility values for 37 HLA-A alleles. The technique was validated by comparing RSCA and sequence-specific oligonucleotide probe (SSOP)/sequence-specific primer amplification (SSP) HLA-A locus typing results from 214 cord blood samples. Of the samples tested, 6.5% required confirmatory typing by SSP, compared with a repeat rate of 10-40% for SSOP. In 200 samples where no SSP was necessary, there was 100% concordance between RSCA and previous results. The ABI PRISM 310 RSCA method defines HLA-A types at medium resolution and is quick and easy to implement.

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Electrophoresis, CapillaryEvaluation Studies as TopicGenetic TechniquesHLA-A AntigensHeteroduplex AnalysisHistocompatibility TestingHumansSequence Analysis, DNAJournal Article

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D M Turner, A Poles, J Brown, J R Argüello, J A Madrigal, C V Navarrete. 1999. HLA-A typing by reference strand-mediated conformation analysis (RSCA) using a capillary-based semi-automated genetic analyser.. https://doi.org/10.1034/j.1399-0039.1999.540410.x

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