The outer envelope protein OEP24 from pea chloroplasts can functionally replace the mitochondrial VDAC in yeast.

T Röhl; M Motzkus; J Soll

doi:10.1016/s0014-5793(99)01399-x

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The outer envelope protein OEP24 from pea chloroplasts can functionally replace the mitochondrial VDAC in yeast.

T Röhl·M Motzkus·J Soll

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Abstract

The chloroplastic outer envelope protein OEP24 from pea forms a high-conductance low specificity solute channel as shown by in vitro studies. In order to establish its function also in an in vivo-like system, the gene encoding OEP24 was transformed into a yeast strain which lacks the general mitochondria solute channel porin, also known as voltage-dependent anion channel (VDAC). Transformation of the yeast VDAC(-) strain with the OEP24 gene resulted in the recovery of a phenotype indistinguishable from the wild-type. The OEP24 polypeptide is targeted to the mitochondrial outer membrane in this heterologous system. We conclude that OEP24 forms a solute channel in pea chloroplasts in planta.

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Chloroplasts Fungal Proteins Intracellular Membranes Ion Channels Mitochondria Pisum sativum Permeability Plant Proteins Porins Saccharomyces cerevisiae Voltage-Dependent Anion Channels Journal Article Research Support, Non-U.S. Gov't

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T Röhl, M Motzkus, J Soll. 1999-11-05. The outer envelope protein OEP24 from pea chloroplasts can functionally replace the mitochondrial VDAC in yeast.. https://doi.org/10.1016/s0014-5793(99)01399-x

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