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PubMed · 10558908

Function of human factor H and I on xenosurface.

M Yoshitatsu·S Miyagawa·S Mikata·K Matsunami·M Yamada·A Murase·Y Sawa·S Ohtake·H Matsuda·R Shirakura
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Abstract

The cell membrane-bound forms of mini-factor H with 1-4 short consensus repeats (fH-PI) and factor I (fI-PI) were constructed. Swine endothelial cell (SEC) lines and Chinese hamster ovary (CHO) cell expressing fH-PI or fI-PI were established and confirmed by flow cytometry. The cell lysate of the SEC line expressing fH-PI showed strong cofactor activity for the cleavage of C3b, and fI-PI demonstrated the protease activity for C4b and C3b not only in the fluid phase but also on the cell membrane. In addition, fH-PI blocked human complement-mediated cell lysis by approximately 30-40%. An SEC line with a low expression of fI-PI showed a weak inhibition of cell lysis in human serum, whereas a CHO cell transfectant with a high expression of fI-PI showed over a 60% inhibition of cell lysis. The results suggest that fH-PI and fI-PI have potential for use in clinical xenotransplantation.

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AnimalsBase SequenceCD55 AntigensCHO CellsCell LineCell MembraneComplement ActivationComplement C3Complement C4Complement Factor HCricetinaeDNA PrimersFibrinogenGraft RejectionHumansModels, BiologicalRecombinant ProteinsSwineTransfectionTransplantation, HeterologousJournal ArticleResearch Support, Non-U.S. Gov't

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M Yoshitatsu, S Miyagawa, S Mikata, K Matsunami, M Yamada, A Murase, Y Sawa, S Ohtake, H Matsuda, R Shirakura. 1999-11-19. Function of human factor H and I on xenosurface.. https://doi.org/10.1006/bbrc.1999.1713

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