PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 10563088

[UTIscreen versus UROQUICK: two semiautomatic systems for bacteriuria detection].

A Milagro·B Moles·A Seoane·J Navascués·M Blasco·J B García-Moya
View original source ↗Export metadata ↓

Abstract

BACKGROUND: The aim of the study is to find a rapid and reliable method for urine screen. With this purpose two semiautomatic systems have been evaluated: UTIscreen and UROQUICK. MATERIAL AND METHODS: We have carried out a prospective study of a total 1,070 urine specimens from patients with suspected urinary infection. Each sample has been inoculated in the two semiautomatic systems and compared with the semiquantitative plate culture as the reference method. RESULTS: Both semiautomatic systems showed similar specificity and sensitivity at an interpretative breakpoint of 30,000 CFU/ml, with a low number of false positives. Uninfected specimens can be reported the same day of reception. CONCLUSIONS: Both UTIscreen and UROQUICK systems seem to be adequate for urinary tract infection screening. They are reliable and easy to perform, but in our particular case we find advantage in using UTIscreen because the faster reading of this system makes posible to report the negative results in the same morning of reception of the specimen and do the culture of all the positive samples during our usual working schedule.

Explore related subjects

BacteriuriaHumansMass ScreeningProspective StudiesReagent Kits, DiagnosticReproducibility of ResultsTime FactorsComparative StudyEnglish AbstractJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

A Milagro, B Moles, A Seoane, J Navascués, M Blasco, J B García-Moya. 1999. [UTIscreen versus UROQUICK: two semiautomatic systems for bacteriuria detection].. https://pubmed.ncbi.nlm.nih.gov/10563088/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2006

Proteus bacteriuria is associated with significant morbidity in spinal cord injury.

STUDY DESIGN: Retrospective chart review. OBJECTIVES: We investigated the morbidity associated with Proteus bacteriuria in a spinal cord injured (SCI) population. SETTING: Michael E DeBakey Veterans Affairs Medical Center in Houston, Texas, USA. METHODS: We reviewed the medical records of all veterans with SCI who received care in our medical center during the past 3 years. Proteus bacteriuria was defined as the growth of Proteus species in any urine culture during the study period. Urinary stones were defined as either renal or bladder calculi. RESULTS: During the study period, 71 of the 501 subjects (14%) had Proteus and 90 (18%) had urinary stones. Twenty-seven percent of the subjects with Proteus had stones, and the association of Proteus with stones was significant (P<0.05). Proteus bacteriuria was likewise associated with complete injury, hospitalization, decubitus ulcers, and history of stones (P<0.001). Subjects using indwelling catheters, either transurethral or suprapubic, were significantly more likely to have Proteus, whereas subjects practising spontaneous voiding and clean intermittent catheterization were significantly less likely to have Proteus. In the 90 patients with stones, Proteus was associated with requiring treatment for stones and having multiple stones (P<0.01). Twenty-five of the 90 patients with stones (28%) required treatment, most often with lithotripsy, and 6 (7%) developed urosepsis. CONCLUSIONS: In persons with SCI, Proteus was found in subjects with a greater degree of impairment who were more likely to be hospitalized, to have decubiti, and to use indwelling catheters. Bacteriuria with Proteus predicted urologic complications in persons with SCI. STATEMENT OF ETHICS: All applicable institutional and governmental regulations concerning the ethical use of human volunteers were followed during the course of this research.

Bacteriuria↗
PubMed2006

Escherichia coli isolates associated with uncomplicated and complicated cystitis and asymptomatic bacteriuria possess similar phylogenies, virulence genes, and O-serogroup profiles.

The genetic and serological characteristics of Escherichia coli isolates from patients with uncomplicated cystitis (UC), complicated cystitis (CC), and complicated asymptomatic bacteriuria (CASB) were determined. Phylogenetic group B2 was predominant in all categories. The prevalences of 14 out of 18 virulence factor genes were similar among the three categories, while pap, iha, ompT, and PAI were more frequently seen in isolates associated with UC than CC or CASB.

Bacteriuria↗
PubMed2006

Use of the BACTEC Mycobacteria Growth Indicator Tube 960 automated system for recovery of Mycobacteria from 9,558 extrapulmonary specimens, including urine samples.

The BACTEC Mycobacteria Growth Indicator Tube 960 (MGIT 960) system was applied for recovery of mycobacteria from extrapulmonary specimens and compared with solid media (Löwenstein-Jensen and Stonebrink). A total of 9,558 specimens were investigated, comprising 3,074 body fluids, 1,878 tissues, and 2,069 urine samples, from which the recovery of mycobacteria was not yet established for MGIT 960. In total, the MGIT 960 was able to detect 446 (90.3%) of the 494 isolates of Mycobacterium tuberculosis complex (MTBC) and 223 (86.0%) out of the 259 isolates of nontuberculous mycobacteria (NTM). In comparison to this, culture on solid medium revealed 358 (72.6%) MTBC isolates and 164 (66.8%) NTM isolates. While 136 (27.6%) of the MTBC isolates and 95 (19.2%) of the NTM isolates were recovered from the MGIT 960 only, 48 (9.7%) of the MTBC isolates and 36 (13.9%) NTM isolates grew only on solid media. Thus, the overall sensitivities for the recovery of mycobacteria from extrapulmonary specimens with MGIT 960 and solid media were 88.8% and 69.3%, respectively. However, the efficiency of the MGIT 960 system can be maximized with additional culture on solid media.

Bacteriuria↗