[Flow cytometry in hematologic diagnosis].

M Pietruczuk; M Dabrowska

Home/Archive/PubMed

PubMed · 10575920

[Flow cytometry in hematologic diagnosis].

M Pietruczuk·M Dabrowska

View original source ↗Export metadata ↓

Abstract

The paper presents flow cytometry method in haematology diagnostics. Beside to the estimation of blast cells phenotype in acute leukaemia, flow cytometry allows to evaluate the presence of minimal residual disease (MRD) and multi drug resistance protein (MDR). Flow cytometry plays a crucial role in the estimation of DNA profile in cancer cells and evaluation of stem cells. Repeatedly flow cytometry is used to estimate platelets and reticulocytes.

Explore related subjects

DNA, Neoplasm Drug Resistance, Multiple Flow Cytometry Hematologic Diseases Leukemia Neoplasms Platelet Count Reticulocyte Count English Abstract Journal Article Review

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

M Pietruczuk, M Dabrowska. 1999. [Flow cytometry in hematologic diagnosis].. https://pubmed.ncbi.nlm.nih.gov/10575920/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2007

Association of interleukin-1 gene polymorphisms with gastric cancer: a meta-analysis.

Previous studies on the association between interleukin-1 (IL-1) genetic polymorphisms and the risk of gastric cancer have produced conflicting results. The purpose of this study was to examine the association between IL-1 genotype and gastric cancer by systematically reviewing the risk of the original studies. Thirty-nine studies, which included 6,863 gastric cancer cases and 8,434 controls, met the inclusion criteria and were included in the meta-analysis. By pooling all the studies identified, the summary odds ratio (OR) of gastric cancer risk associated with IL-1B-511T, -31C, +3954T and IL-1RN*2 was 1.26 (95% confidence interval (CI): 1.03-1.55), 1.00 (95% CI: 0.82-1.22), 1.37 (95% CI: 0.94-2.00) and 1.20 (95% CI: 1.01-1.41), respectively. A stratified analysis showed that IL-1B-511T was associated with an increased risk of gastric cancer (intestinal type) (OR = 1.76, 95% CI: 1.12-2.57). Moreover, IL-1RN*2 was also associated with an increased risk of gastric cancer among Caucasians (OR = 1.30, 95% CI: 1.09-1.54). In conclusion, IL-1B-511 and IL-1RN genetic polymorphisms are associated with an increased risk of developing gastric cancer.

DNA, Neoplasm↗

PubMed2007

Congenital linear unilateral basal cell nevus: a case report with patched gene molecular studies.

BACKGROUND: Linear unilateral basal cell nevus represents a linear collection of macules and papules histologically similar to basal cell carcinoma but with benign clinical behavior. We describe a patient who initially presented at the age of 6 months with a unilateral linear basal cell nevus on the right flank. The differential diagnosis included the nevoid basal cell carcinoma syndrome. Constitutional PTCH mutations are causative of the nevoid basal cell carcinoma syndrome, and somatic PTCH mutations are found in the vast majority of basal cell carcinomas. Somatic SMO mutations have also been found in some basal cell carcinomas. METHODS: Histologic examination of the lesions is performed. Short tandem-repeat molecular analysis at the PTCH locus and sequencing of PTCH and SMO genes is performed. RESULTS: Histologic examination revealed features initially indistinguishable from basal cell carcinoma. Short tandem-repeat DNA analysis did not reveal loss of heterozygosity at the PTCH locus. DNA sequencing of both the PTCH and the SMO genes from the patient's lesions revealed neither inactivating mutations of PTCH nor activating mutations of SMO. CONCLUSION: Molecular examination indicates that the PTCH and SMO genes are not involved in the pathogenesis of the patients' congenital linear unilateral basal cell nevus. Furthermore, we discuss the relationship between linear basal cell nevus and basaloid follicular hamartoma.

DNA, Neoplasm↗

PubMed2006

Homeobox genes and their functions on development and neoplasm in gastrointestinal tract.

AIM: To describe the role of homeobox genes on development and tumorigenesis in gastrointestinal tract. METHODS AND RESULTS: We searched the MEDLINE database (until March, 2006) with the keywords of homeobox genes, gastrointestinal tract, development, tumorigenesis, carcinogenesis and therapeutic targets. We reviewed the literature on classification of homeobox genes, development of gastrointestinal tract, carcinogenesis of gastrointestinal tract as well as therapeutic targets. CONCLUSIONS: The functional effects of homeobox family in development and tumorigenesis of gastrointestinal tract are identified. The importance of homeobox genes and a possibility of therapeutic intervention in clinical medicine are discussed.

DNA, Neoplasm↗