PubMed · 10583246
The source did not provide an abstract. Follow the original record for more information.
Explore connections, maps & timelines
R Anwar, K J Miloszewski. 1999. Factor XIII deficiency.. https://doi.org/10.1046/j.1365-2141.1999.01648.x
Cite the original work for its findings. Save a collection to share your selection of sources.
KEEP EXPLORING
Cross-linking mass spectrometry is a powerful method for structural analysis, but choosing between cleavable and non-cleavable cross-linkers remains challenging. We rigorously compared non-cleavable DSS with cleavable DSSO and found that DSS consistently yields more cross-link identifications from isolated protein complexes to bacterial lysates. The advantage of DSS diminishes as sample complexity increases. At the highest complexity tested-human cell lysate-the trend reverses, with DSSO outperforming DSS. The superior performance of DSS in less complex samples is likely explained by its longer and more flexible spacer arm, which interrogates a spatial volume >40% larger than that of DSSO. For both cross-linkers, the number of identified cross-links decreases as the search space expands, but more steeply for DSS. This sharper decline arises from DSS cross-links producing slightly lower fragment ion coverage, not from the absence of signature ions that could reduce search space. Fragment ion coverage is key to interactome mapping: when coverage reaches 85% or above, identification sensitivity hardly decreases as the search space expands, regardless of the cross-linker used. In summary, we recommend DSS for samples no more complex than bacterial lysates. For interactome mapping of mammalian cells, although DSSO outperforms DSS, neither achieves deep interactome coverage.
New synthetic chemical inducers of dimerization, comprising homodimeric FKBP ligands with engineered specificity for the designed point mutant F36V, have been evaluated for inducing targeted gene expression in mammalian cells. Structure-activity studies indicated that high-affinity dimerizers such as AP1903 are ineffective, perhaps due to kinetic trapping of non-productive dimers, whereas lower-affinity molecules, exemplified by AP1889 and AP1966, potently activate transcription.
[structure: see text] A convenient approach to a series of star-shaped pi-conjugated organic materials with oligofluorenes (OF) as the branches and a benzene ring as the core has been developed. The optical properties of these materials are liable to be tuned by introduction of more fluorene rings. The investigation of the structure-property relationship of these materials has indicated differences between star-shaped oligofluorenes and linear oligofluorenes, as well as polyfluorenes.