A simple estimation method for the embryonic toxicity of plastic wrapping sheets using an egg shell-free culture system of chick embryos.

Y Okai; K Higashiokai

doi:10.7888/juoeh.21.191

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A simple estimation method for the embryonic toxicity of plastic wrapping sheets using an egg shell-free culture system of chick embryos.

Y Okai·K Higashiokai

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Abstract

To estimate the embryonic toxicity of food wrapping films, the shell-free chick embryos were cultured using plastic wrapping sheets like a suspending hammock. Polyethylene (PE) wrapping sheets with no additives showed much higher survivability than polyvinylchloride (PVC) wrapping sheets including plasticizers. Furthermore when a PVC wrapping sheet was autoclaved at 110 degrees C for 30 min, the embryonic survivability significantly decreased compared with the untreated PVC wrapping sheet. These observations suggest that the egg shell-free culture method using a plastic wrapping sheet is a useful estimation method for the embryonic toxicity of food wrapping film.

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Animals Chick Embryo Culture Techniques Food Packaging Plasticizers Polyethylene Polyvinyl Chloride Temperature Time Factors Tissue Survival Toxicity Tests Journal Article

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BibTeX RIS

Y Okai, K Higashiokai. 1999-09-01. A simple estimation method for the embryonic toxicity of plastic wrapping sheets using an egg shell-free culture system of chick embryos.. https://doi.org/10.7888/juoeh.21.191

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