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PubMed · 105900

Simultaneous competitive enzyme immunoassay for human chorionic gonadotropin.

K Hamada·S Tomoda·T Sugawa·K P Takahashi
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Abstract

A simultaneous competitive enzyme immunoassay (SICEIA) for hCG was developed using beta-D-galactosidase (beta-Gal) as a labelled enzyme and anti-hCG antibody coated sheep red blood cells (SRBC) as a solid phase. In this report, a new coupling agent, MCAE, was used to couple beta-Gal with hCG. The sensitivity was improved to the degree of 2.5 mIU/ml, equal to that of RIA. The present procedure was safer and rapider than RIA. The value of hCG in urine by our procedure had good correlation with that by RIA.

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Binding, CompetitiveChorionic GonadotropinHumansImmunoenzyme Techniquesbeta-GalactosidaseJournal Article

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BibTeXRIS

K Hamada, S Tomoda, T Sugawa, K P Takahashi. 1978. Simultaneous competitive enzyme immunoassay for human chorionic gonadotropin.. https://doi.org/10.1507/endocrj1954.25.515

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