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PubMed · 10603311

Tn5: A molecular window on transposition.

W S Reznikoff·A Bhasin·D R Davies·I Y Goryshin·L A Mahnke·T Naumann·I Rayment·M Steiniger-White·S S Twining
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Abstract

DNA transposition is an underlying process involved in the remodeling of genomes in all types of organisms. We analyze the multiple steps in cut-and-paste transposition using the bacterial transposon Tn5 as a model. This system is particularly illuminating because of the existence of structural, genetic, and biochemical information regarding the two participating specific macromolecules: the transposase and the 19-bp sequences that define the ends of the transposon. However, most of the insights should be of general interest because of similarities to other transposition-like systems such as HIV-1 DNA integration into the host genome.

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Base SequenceDNADNA Transposable ElementsProtein BindingTransposasesJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.Review

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W S Reznikoff, A Bhasin, D R Davies, I Y Goryshin, L A Mahnke, T Naumann, I Rayment, M Steiniger-White, S S Twining. 1999-12-29. Tn5: A molecular window on transposition.. https://doi.org/10.1006/bbrc.1999.1891

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