Conformational transition in DNA on a cold surface.

X Z Feng; R Bash; P Balagurumoorthy; D Lohr; R E Harrington; S M Lindsay

doi:10.1093/nar/28.2.593

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Conformational transition in DNA on a cold surface.

X Z Feng·R Bash·P Balagurumoorthy·D Lohr·R E Harrington·S M Lindsay

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Abstract

The contour length of DNA fragments, deposited and imaged on mica under buffer, was measured as a function of deposition temperature. Extended DNA molecules (on Ni- and silane-treated surfaces) contract rapidly with falling temperature, approaching the contour length of A-DNA at 2 degrees C. The contraction is not unique to a specific sequence and does not occur in solution at 2 degrees C or on a surface at 25 degrees C, indicating that it arises from a combination of low temperature and surface contact. It is probably a consequence of reduced water activity at a cold surface.

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Aluminum Silicates Circular Dichroism Cold Temperature DNA, Viral Microscopy, Atomic Force Nucleic Acid Conformation Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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X Z Feng, R Bash, P Balagurumoorthy, D Lohr, R E Harrington, S M Lindsay. 2000-01-15. Conformational transition in DNA on a cold surface.. https://doi.org/10.1093/nar%2F28.2.593

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