Towards single-copy gene expression systems making gene cloning physiologically relevant: lambda InCh, a simple Escherichia coli plasmid-chromosome shuttle system.

D Boyd; D S Weiss; J C Chen; J Beckwith

doi:10.1128/jb.182.3.842-847.2000

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Towards single-copy gene expression systems making gene cloning physiologically relevant: lambda InCh, a simple Escherichia coli plasmid-chromosome shuttle system.

D Boyd·D S Weiss·J C Chen·J Beckwith

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Abstract

We describe a simple system for reversible, stable integration of plasmid-borne genes into the Escherichia coli chromosome. Most ordinary E. coli strains and a variety of pBR322-derived ampicillin-resistant plasmids can be used. A single genetic element, a lambda phage, is the only specialized vector required. The resultant strains have a single copy of the plasmid fragment inserted stably at the lambda attachment site on the chromosome, with nearly the entire lambda genome deleted.

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Ampicillin Resistance Bacteriophage lambda Chromosomes, Bacterial Cloning, Molecular Escherichia coli Gene Expression Regulation, Bacterial Gene Transfer Techniques Genetic Vectors Plasmids Journal Article

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D Boyd, D S Weiss, J C Chen, J Beckwith. 2000. Towards single-copy gene expression systems making gene cloning physiologically relevant: lambda InCh, a simple Escherichia coli plasmid-chromosome shuttle system.. https://doi.org/10.1128/jb.182.3.842-847.2000

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