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PubMed · 10634481

DNA sequencing in a monolithic microchannel device.

C Backhouse·M Caamano·F Oaks·E Nordman·A Carrillo·B Johnson·S Bay
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Abstract

We present 50 cm long microchannels in a monolithic device for high resolution, long read-length DNA sequencing. These devices were fabricated and bonded in borofloat glass using unconventional photolithography techniques with 48-188 independent, straight microchannels. The microchannel DNA separation was tested with POP-6 polymer and a DNA sequencing ladder separated at room temperature and 200 V/cm. Single-base resolution greater than 600 bases was achieved and the sequence base called to 640 bases with 98% accuracy. Under the same experimental conditions, the performance of the microchip was identical to a fused-silica capillary with similar cross-sectional area.

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AnimalsBase SequenceDNAElectrophoresisEquipment and SuppliesHumansMolecular Sequence DataSequence Analysis, DNAJournal Article

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C Backhouse, M Caamano, F Oaks, E Nordman, A Carrillo, B Johnson, S Bay. 2000. DNA sequencing in a monolithic microchannel device.. https://doi.org/10.1002/(sici)1522-2683(20000101)21%3A1%3C150%3A%3Aaid-elps150%3E3.0.co%3B2-5

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