PubMed · 10635932
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K Kitamura. 1999. [Cross matching test].. https://pubmed.ncbi.nlm.nih.gov/10635932/
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HYPOTHESIS: Recipients of 0 HLA mismatch kidneys with prolonged cold ischemia times of longer than 36 hours do not have superior outcomes compared with recipients of kidneys with 1 or more mismatches. DESIGN: Retrospective review. SETTING: Transplanation centers. PATIENTS AND METHODS: A total of 63,688 recipients who underwent transplantation between January 1, 1990, and July 31, 1998. MAIN OUTCOME MEASURES: Delayed graft function, serum creatinine level, and patient and renal graft survival. RESULTS: Recipients of 0 HLA mismatch kidneys with fewer than 36 hours of cold ischemia time had better 5-year graft survival (75%) when compared with recipients with 1 or more mismatches (67%) (P<.001). However, recipients of 0 HLA mismatch kidneys with longer than 36 hours of cold ischemia time did not have any graft survival advantage (71% in 0 HLA mismatch kidneys vs 72% in 1 or more mismatches, P =.24). CONCLUSIONS: Cold ischemia times of longer than 36 hours obviate the benefits of better graft survival conferred by better matching.
The ultrasonic wave action upon immune red blood cell (RBC) aggregation in vitro was investigated by means of the elastic light-scattering method. The RBC agglutination process in the ultrasonic wave presence was analysed as a function of irradiation time, the antiserum series, the whole blood and antiserum dilution degree and blood group types. It was experimentally shown that the ultrasonic irradiation accelerated the agglutination reaction in the blood and antiserum mixture. The sedimentation process showed a gain in the resolution of the optical method's ability to detect the induced RBC complex formation. It revealed that the ultrasonic application led to significant diagnostic test time shortening. The results may be used to develop new diagnostic techniques and devices.