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PubMed · 10648930

Selectively infective phage (SIP) technology: scope and limitations.

S Jung·K M Arndt·K M Müller·A Plückthun
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Abstract

We review here the selectively infective phage (SIP) technology, a powerful tool for the rapid selection of protein-ligand and peptide-ligand pairs with very high affinities. SIP is highly suitable for discriminating between molecules with subtle stability and folding differences. We discuss the preferred types of applications for this technology and some pitfalls inherent in the in vivo SIP method that have become apparent in its application with highly randomized libraries, as well as some precautions that should be taken in successfully applying this technology.

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Bacteriophage M13Capsid ProteinsDNA-Binding ProteinsHumansPeptide LibraryRecombination, GeneticViral Fusion ProteinsJournal ArticleReview

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BibTeXRIS

S Jung, K M Arndt, K M Müller, A Plückthun. 1999-12-10. Selectively infective phage (SIP) technology: scope and limitations.. https://doi.org/10.1016/s0022-1759(99)00143-x

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