An indexed genomic library for Paramecium complementation cloning.

A M Keller; J Cohen

doi:10.1111/j.1550-7408.2000.tb00001.x

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An indexed genomic library for Paramecium complementation cloning.

A M Keller·J Cohen

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Abstract

Recent pioneering work opened the way for cloning genes in Paramecium by functional complementation of mutants. We present here the construction and pilot utilization of a new indexed library of Paramecium macronuclear DNA. The library is made of 61,440 clones containing inserts mostly between 6 and 12 kilobases. It has already allowed the complementation cloning of four new genes, and this library has proven to be very useful for rapid hybridization cloning.

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Animals Cloning, Molecular DNA, Protozoan Genetic Complementation Test Genomic Library Nucleic Acid Hybridization Paramecium Journal Article Research Support, Non-U.S. Gov't

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A M Keller, J Cohen. An indexed genomic library for Paramecium complementation cloning.. https://doi.org/10.1111/j.1550-7408.2000.tb00001.x

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