A bacterial high-affinity GABA binding protein: isolation and characterization.

G D Guthrie; C S Nicholson-Guthrie; H L Leary

doi:10.1006/bbrc.1999.1960

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A bacterial high-affinity GABA binding protein: isolation and characterization.

G D Guthrie·C S Nicholson-Guthrie·H L Leary

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Abstract

A gamma-aminobutyric acid (GABA) binding protein (GBP) was isolated from a bacterial mutant which has high-affinity GABA binding characteristics comparable with the GABA(A) brain receptor in mammals. The GBP was partially purified and characterized and was shown to be a periplasmic protein of approximately 42,000 molecular weight. To determine the molecular weight, a bacterial GABA binding assay was used with SDS-PAGE. This procedure did not require large amounts or complete purification of protein and may be useful as a simple method in estimating the molecular weight of other bacterial binding proteins.

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Animals Bacterial Proteins Binding, Competitive Carrier Proteins Mammals Molecular Weight Mutation Pseudomonas fluorescens Receptors, GABA-A gamma-Aminobutyric Acid Comparative Study Journal Article Research Support, Non-U.S. Gov't

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G D Guthrie, C S Nicholson-Guthrie, H L Leary. 2000-02-05. A bacterial high-affinity GABA binding protein: isolation and characterization.. https://doi.org/10.1006/bbrc.1999.1960

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