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PubMed · 10666443

MethTools--a toolbox to visualize and analyze DNA methylation data.

C Grunau·R Schattevoy·N Mache·A Rosenthal
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Abstract

The Bisulfite Genomic Sequencing technique has found wide acceptance for the generation of DNA-methylation maps with single-base resolution. The method is based on the selective deamination of cytosine to uracil (and subsequent conversion to thymine via PCR), whereas 5-methylcytosine residues remain unchanged. Methylation maps are created by the comparison of bisulfite converted sequences with the untreated genomic sequence. 'MethTools' is a collection of software tools that replaces the time-consuming manual comparison process, generates graphical outputs of methylation patterns and methyl-ation density, estimates the systematic error of the experiment and searches for conserved methylated nucleotide patterns. The programs are written in Perl 5 and C, and the source code can be downloaded. All tools run independently but the programs are interfaced. Thus, a script can perform the entire analysis procedure automatically. In addition, a web-based remote analysis service is offered. Both the source code and the remote analysis are available at http://genome.imb-jena.de/methtools/

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AnimalsDNA MethylationHumansSoftwareStatistics as TopicJournal ArticleResearch Support, Non-U.S. Gov't

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C Grunau, R Schattevoy, N Mache, A Rosenthal. 2000-03-01. MethTools--a toolbox to visualize and analyze DNA methylation data.. https://doi.org/10.1093/nar%2F28.5.1053

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