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CD11b/CD18-coated microspheres attach to E-selectin under flow.

K L Crutchfield·V R Shinde Patil·C J Campbell·C A Parkos·J R Allport·D J Goetz
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Abstract

Neutrophils can attach to E-selectin under flow. Proposed ligands for E-selectin carry SLe(x)-type glycans. The leukocyte beta2 integrins are glycosylated with SLe(x). Thus, we speculated that beta2 integrins could support attachment to E-selectin. To test this hypothesis, we coated 10-microm-diameter microspheres with purified CD11b/CD18 (alphaMbeta2) and investigated the adhesion of the resulting alphaMbeta2 microspheres to E-selectin. Under in vitro flow conditions, the alphaMbeta2 microspheres attached to Chinese hamster ovary cells expressing E-selectin (CHO-E) and 4-h interleukin-1beta-activated human umbilical vein endothelial cells (HUVEC). At a shear stress of 1.8 dynes/cm2, the attachment events were eliminated by pretreatment of the cellular monolayers with a mAb to E-selectin. alphaMbeta2 microspheres did not attach to untransfected CHO cells or unactivated HUVEC at 1.8 dynes/cm2. Taken together, the results strongly suggest that the CD11b/CD18-E-selectin bond has sufficient biophysical properties to mediate attachment of neutrophil-sized particles to E-selectin under flow.

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AnimalsAntibodies, MonoclonalCD18 AntigensCHO CellsCell AdhesionCells, CulturedCricetinaeCricetulusE-SelectinEndothelium, VascularFibrinogenGangliosidesGlycosylationHumansMacrophage-1 AntigenMicrospheresNeuraminidaseProtein Processing, Post-TranslationalRecombinant Fusion ProteinsRheologySialyl Lewis X AntigenTransfectionUmbilical VeinsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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K L Crutchfield, V R Shinde Patil, C J Campbell, C A Parkos, J R Allport, D J Goetz. 2000. CD11b/CD18-coated microspheres attach to E-selectin under flow.. https://doi.org/10.1002/jlb.67.2.196

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