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Age-dependent increase in C7-1 gene expression in rat frontal cortex.

H Hung·M J Tsai·H C Wu·E H Lee
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Abstract

We have previously reported that a fragment of mRNA, denoted as C7-1, which expression was significantly increased in the frontal cortex of aged rats. In the present study, we have cloned and sequenced the full length cDNA of the C7-1 gene. We have found that the open reading frame of this gene encoded a 463-amino-acid protein, which shared 84% identity in amino acid sequence with a subunit of vacuolar H(+)-ATPase (V-ATPase). Further Northern blot analysis revealed that there was an age-dependent increase in C7-1 gene expression in rat frontal cortex, but not in other brain areas. Moreover, application of C7-1 antisense oligonucleotide to cortical neuronal cultures markedly inhibited cell survival. These results together suggest that C7-1 is a marker for the aging process and that upregulation of C7-1 may be important in maintaining the normal function of V-ATPase during aging.

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AgingAmino Acid SequenceAnimalsBase SequenceBiomarkersBlotting, NorthernCell SurvivalCells, CulturedCloning, MolecularDNA, ComplementaryGene Expression RegulationIn Vitro TechniquesMaleMolecular Sequence DataNeuronsOligonucleotides, AntisensePrefrontal CortexProtein BiosynthesisProteinsProton-Translocating ATPasesRNA, MessengerRatsRats, Sprague-DawleySequence Homology, Amino AcidTissue DistributionVacuolar Proton-Translocating ATPasesJournal ArticleResearch Support, Non-U.S. Gov't

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H Hung, M J Tsai, H C Wu, E H Lee. 2000-02-22. Age-dependent increase in C7-1 gene expression in rat frontal cortex.. https://doi.org/10.1016/s0169-328x(99)00325-3

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