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PubMed · 10694907

Microwave processing for scanning electron microscopy.

F Hernández·R Guillén
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Abstract

The normal processing of biological samples for Scanning Electron Microscopy, includes treatment with aldehyde (1 to 2 hours), postfixation with Osmium (1 hour), followed by dehydration in a ascending grade of ethanol (30 a 100%), 10 to 15 minutes in each step, and finally drying. This procedure takes at least 8 hours. In this work, samples of mosquitoes (Aedes), protozoa (Tritrichomonas muris), bacteria (Clostridium oceanicum), murine liver, and small intestine were processed in the same manner in a domestic microwave oven for two minutes at 20% of its maximum power. The complete procedure from the initial fixation to dehydration in 100% ethanol was reduced to one hour with good preservation of the ultrastructural details of the specimens.

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AedesAnimalsClostridiumDesiccationIntestine, SmallLiverMiceMicroscopy, Electron, ScanningMicrowavesSpecimen HandlingTissue FixationJournal ArticleResearch Support, Non-U.S. Gov't

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F Hernández, R Guillén. 2000. Microwave processing for scanning electron microscopy.. https://pubmed.ncbi.nlm.nih.gov/10694907/

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