Transforming growth factor beta 1 (TGF beta 1) down-regulates expression and function of proliferation-inducing molecules in HPV-transformed cells.

M K Shier; E B Neely; M G Ward; C Meyers; M K Howett

PubMed · 10697499

Transforming growth factor beta 1 (TGF beta 1) down-regulates expression and function of proliferation-inducing molecules in HPV-transformed cells.

M K Shier·E B Neely·M G Ward·C Meyers·M K Howett

Abstract

The epidemiologic association of human papillomavirus (HPV) infection with dysplasia and cervical cancer is well established. Transforming growth factor beta 1 (TGF beta 1) is a growth inhibitory protein for epithelial cells. To examine the phenotype of HPV-transformed cells, we examined expression of TGF beta 1 and a number of cellular proliferation-enhancing molecules which are known to be regulated by TGF beta 1, including bcl-2, c-jun and NFkB. Previous studies had identified significant induction of TGF beta 1 and concomitant down-regulation of other growth stimulatory molecules in experimental papillomas. We used HPV-16 and -18 transformed cell lines. The HPV-16 transformed cells showed down-regulation of bcl-2 and NFkB as well as NFkB function upon TGF beta 1 treatment. The results suggest that TGF beta 1 may exert antiproliferative effects on some HPV-transformed cells by down-regulating expression and function of different proliferation-enhancing molecules. It is uncertain if this function is virus type specific and/or related to state of tumor cell progression.

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Base Sequence Cell Division Cell Transformation, Viral Cells, Cultured DNA Primers Down-Regulation Humans Papillomaviridae Proto-Oncogene Proteins c-bcl-2 Proto-Oncogene Proteins c-jun Reverse Transcriptase Polymerase Chain Reaction Transforming Growth Factor beta Up-Regulation Journal Article Research Support, U.S. Gov't, P.H.S.

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M K Shier, E B Neely, M G Ward, C Meyers, M K Howett. Transforming growth factor beta 1 (TGF beta 1) down-regulates expression and function of proliferation-inducing molecules in HPV-transformed cells.. https://pubmed.ncbi.nlm.nih.gov/10697499/

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