PubMed Health⌕ Search

PubMed · 10698651

Diffusion measurements using the nonlinear stimulated echo.

Abstract

The nonlinear stimulated echo that is generated by a sequence of three radiofrequency pulses, 90 degrees-tau(1)-90 degrees-tau(2)-45 degrees, in high magnetic fields (or at low temperatures) in the presence of pulsed or steady field gradients can be applied for measurements of the diffusion coefficient. Corresponding test experiments are reported. Steady gradients can be used without knowledge of the relaxation times. Remarkably the attenuation of the nonlinear stimulated echo by diffusion is substantially stronger than in the case of the ordinary stimulated echo.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

I Ardelean, R Kimmich. 2000. Diffusion measurements using the nonlinear stimulated echo.. https://doi.org/10.1006/jmre.1999.1975

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

Novel distance dependence of diffusion constants in hyaluronan aqueous solution resulting from its characteristic nano-microstructure.

Material transports in hyaluronan (HA) aqueous solution were investigated applying two different techniques, i.e., pulsed field gradient NMR (PFG-NMR) and photochemical quenching, to the measurement of diffusion constants to show a sharp contrast resulting from the difference of the spectroscopic observation time while the same probe molecules were commonly used in two experiments. The value from PFG-NMR reflects the relatively long transport along which the majority of the molecules are retarded by the mesh structure of HA solution. In such inhomogeneous fluids, the observable diffusion constant should generally depend on the observation time and, i.e., the averaged distance of diffusion. Quantitative discussion, which compares the obtained characteristic distance of diffusion with the pore size, clarifies the role of the nano-microstructure of HA solution forming small pores surrounded by the polymer chain networks.

Diffusion↗

Diffusion of nitric oxide into low density lipoprotein.

A key early event in the development of atherosclerosis is the oxidation of low density lipoprotein (LDL) via different mechanisms including free radical reactions with both protein and lipid components. Nitric oxide (( small middle dot)NO) is capable of inhibiting LDL oxidation by scavenging radical species involved in oxidative chain propagation reactions. Herein, the diffusion of ( small middle dot)NO into LDL is studied by fluorescence quenching of pyrene derivatives. Selected probes 1-(pyrenyl)methyltrimethylammonium (PMTMA) and 1-(pyrenyl)-methyl-3-(9-octadecenoyloxy)-22,23-bisnor-5-cholenate (PMChO) were chosen so that they could be incorporated at different depths of the LDL particle. Indeed, PMTMA and PMChO were located in the surface and core of LDL, respectively, as indicated by changes in fluorescence spectra, fluorescence quenching studies with water-soluble quenchers and the lifetime values (tau(o)) of the excited probes. The apparent second order rate quenching constants of ( small middle dot)NO (k(NO)) for both probes were 2.6-3.8 x 10(10) m(-1) s(-1) and 1.2 x 10(10) m(-1) s(-1) in solution and native LDL, respectively, indicating that there is no significant barrier to the diffusion of ( small middle dot)NO to the surface and core of LDL. Nitric oxide was also capable of diffusing through oxidized LDL. Considering the preferential partitioning of ( small middle dot)NO in apolar milieu (6-8 for n-octanol:water) and therefore a larger ( small middle dot)NO concentration in LDL with respect to the aqueous phase, a corrected k(NO) value of approximately 0.2 x 10(10) m(-1) s(-1) can be determined, which still is sufficiently large and consistent with a facile diffusion of ( small middle dot)NO through LDL. Applying the Einstein-Smoluchowsky treatment, the apparent diffusion coefficient (D(')NO) of ( small middle dot)NO in native LDL is on average 2 x 10(-5) cm(2) s(-1), six times larger than that previously reported for erythrocyte plasma membrane. Thus, our observations support that ( small middle dot)NO readily traverses the LDL surface accessing the hydrophobic lipid core of the particle and affirm a role for ( small middle dot)NO as a major lipophilic antioxidant in LDL.

Diffusion↗

Kinetics of the coupled reaction catalysed by a fusion protein of beta-galactosidase and galactose dehydrogenase.

The mechanistic implications of the kinetic behaviour of a fusion protein of beta-galactosidase and galactose dehydrogenase have been analysed in view of predictions based on experimentally determined kinetic parameter values for the galactosidase and dehydrogenase activities of the protein. The results show that the time course of galactonolactone formation from lactose in the coupled reaction catalysed by the fusion protein can be most satisfactorily accounted for in terms of a free-diffusion mechanism when consideration is given to the mutarotation of the reaction intermediate galactose. It is concluded that no tenable kinetic evidence is available to support the proposal that the fusion protein catalyses galactonolactone formation from lactose by a mechanism involving channelling of galactose.

Diffusion↗