Micro- and macrochromosome paints generated by flow cytometry and microdissection: tools for mapping the chicken genome.

D K Griffin; F Haberman; J Masabanda; P O'Brien; M Bagga; A Sazanov; J Smith; D W Burt; M Ferguson-Smith; J Wienberg

doi:10.1159/000015449

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Micro- and macrochromosome paints generated by flow cytometry and microdissection: tools for mapping the chicken genome.

D K Griffin·F Haberman·J Masabanda·P O'Brien·M Bagga·A Sazanov·J Smith·D W Burt·M Ferguson-Smith·J Wienberg

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Abstract

Despite the chicken being one of the most genetically mapped of all animals, its karyotype remains poorly defined. This is primarily due to microchromosomes that belie assignment by conventional methods. To address this problem, we have developed chromosome-specific paints using flow cytometry and microdissection. For the microchromosomes it was necessary to amplify and label DNA from single microdissected chromosomes.

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Animals Cells, Cultured Chick Embryo Chickens Chromosome Painting Cloning, Molecular Cosmids DNA Probes Flow Cytometry Geese Genome Karyotyping Oligodeoxyribonucleotides Physical Chromosome Mapping Polymerase Chain Reaction Species Specificity Journal Article

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D K Griffin, F Haberman, J Masabanda, P O'Brien, M Bagga, A Sazanov, J Smith, D W Burt, M Ferguson-Smith, J Wienberg. 1999. Micro- and macrochromosome paints generated by flow cytometry and microdissection: tools for mapping the chicken genome.. https://doi.org/10.1159/000015449

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