Thyroid hormone-dependent metamorphosis in a direct developing frog.

E M Callery; R P Elinson

doi:10.1073/pnas.050501097

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PubMed · 10706622

Thyroid hormone-dependent metamorphosis in a direct developing frog.

E M Callery·R P Elinson

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Abstract

The direct developing anuran, Eleutherodactylus coqui, lacks a tadpole, hatching as a tiny frog. We investigated the role of the metamorphic trigger, thyroid hormone (TH), in this unusual ontogeny. Expression patterns of the thyroid hormone receptors, TRalpha and TRbeta, were similar to those of indirect developers. TRbeta mRNA levels increased dramatically around the time of thyroid maturation, when remodeling events reminiscent of metamorphosis occur. Treatment with the goitrogen methimazole inhibited this remodeling, which was reinitiated on cotreatment with TH. Despite their radically altered ontogeny, direct developers still undergo a TH-dependent metamorphosis, which occurs before hatching. We propose a new model for the evolution of anuran direct development.

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Animals Animals, Newborn Antithyroid Agents Bufonidae Cloning, Molecular Embryo, Nonmammalian Immunohistochemistry Metamorphosis, Biological Methimazole Molecular Sequence Data Receptors, Thyroid Hormone Reverse Transcriptase Polymerase Chain Reaction Ribosomal Proteins Thyroid Hormones Time Factors Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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E M Callery, R P Elinson. 2000-03-14. Thyroid hormone-dependent metamorphosis in a direct developing frog.. https://doi.org/10.1073/pnas.050501097

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