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SAC3 may link nuclear protein export to cell cycle progression.

A L Jones·B B Quimby·J K Hood·P Ferrigno·P H Keshava·P A Silver·A H Corbett
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Abstract

Selective movement of proteins between the nucleus and the cytoplasm is a regulatory mechanism exploited extensively by the eukaryotic cell. We have identified the evolutionarily conserved Sac3 protein, which was implicated previously in the regulation of mitosis [Bauer, A. & Kölling, R. (1996) J. Cell Sci. 109, 1575-1583] as a novel mediator of nuclear protein export. We show that Sac3p is localized to the nuclear pore, where it interacts with nucleoporins. Loss of SAC3 function results in a block in nuclear export of a nuclear export signal-containing reporter protein. Our results also demonstrate that SAC3 interacts genetically with the nuclear protein export factors Crm1p/Xpo1p and Yrb2p. Taken together, these data indicate a link between nuclear protein export and transition through the cell cycle.

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Amino Acid SequenceBiological TransportCalcium-Binding ProteinsCell CycleFluorescent Antibody Technique, IndirectFungal ProteinsGene DeletionGreen Fluorescent ProteinsLuminescent ProteinsMolecular Sequence DataNuclear Pore Complex ProteinsNuclear ProteinsNucleocytoplasmic Transport ProteinsPorinsProtein BindingRecombinant Fusion ProteinsSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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A L Jones, B B Quimby, J K Hood, P Ferrigno, P H Keshava, P A Silver, A H Corbett. 2000-03-28. SAC3 may link nuclear protein export to cell cycle progression.. https://doi.org/10.1073/pnas.97.7.3224

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