A simple method for human peripheral blood monocyte isolation.

M C de Almeida; A C Silva; A Barral; M Barral Netto

doi:10.1590/s0074-02762000000200014

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A simple method for human peripheral blood monocyte isolation.

M C de Almeida·A C Silva·A Barral·M Barral Netto

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Abstract

We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.

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Cell Separation Centrifugation, Density Gradient Humans Monocytes Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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M C de Almeida, A C Silva, A Barral, M Barral Netto. A simple method for human peripheral blood monocyte isolation.. https://doi.org/10.1590/s0074-02762000000200014

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