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PubMed · 10739138

Submicrosecond real-time fluorescence sampling: application to protein folding.

J Ervin·J Sabelko·M Gruebele
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Abstract

Time-resolved fluorescence detection has become a central tool in the study of protein folding. This article briefly reviews modern fluorescence techniques and then focuses on recent improvements made possible by array photomultipliers, computer-controlled data gating, and long-memory multi-channel digitizers. It is now possible to detect fluorescence wavelength profiles and/or fluorescence decay transients very cost effectively with sub-microsecond kinetic time resolution out to long times. Folding kinetics can be analyzed by singular value decomposition (SVD) or chi-analysis. The latter provides an objective method for detecting nonexponential kinetics in two-state systems.

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Equipment DesignProtein FoldingProteinsSpectrometry, FluorescenceJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.Review

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BibTeXRIS

J Ervin, J Sabelko, M Gruebele. 2000. Submicrosecond real-time fluorescence sampling: application to protein folding.. https://doi.org/10.1016/s1011-1344(00)00002-6

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