Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control: I. DNA fragment insertion at the lacZ EcoRI restriction site.

C Pourcel; C Marchal; A Louise; A Fritsch; P Tiollais

doi:10.1007/bf00337792

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Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control: I. DNA fragment insertion at the lacZ EcoRI restriction site.

C Pourcel·C Marchal·A Louise·A Fritsch·P Tiollais

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Abstract

Bacteriophage lambda vectors, derived from lambda plac5 were constructed. Their genomes have only one EcoRI restriction site, located near the end of the beta-galactosidase gene. Recombinants, constructed in vitro, having a DNA fragment inserted in the EcoRI site, are lac- and can be easily recognized. Expression of such foreign genes is then under the control of the lac promoter. Mutations Qam73 and Sam7 greatly increase the amount of beta-galactosidase synthesized by the vector bacteriophage. The lambda ZEQS vector has been certified B2 (EK2) by the French control commission "Recombinaisons génétiques in vitro".

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Coliphages DNA, Recombinant DNA, Viral Electrophoresis, Polyacrylamide Gel Escherichia coli Galactosidases Genes, Viral Lac Operon beta-Galactosidase Journal Article

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C Pourcel, C Marchal, A Louise, A Fritsch, P Tiollais. 1979-02-26. Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control: I. DNA fragment insertion at the lacZ EcoRI restriction site.. https://doi.org/10.1007/bf00337792

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