Single-cell MALDI: a new tool for direct peptide profiling.

L Li; R W Garden; J V Sweedler

doi:10.1016/s0167-7799(00)01427-x

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Single-cell MALDI: a new tool for direct peptide profiling.

L Li·R W Garden·J V Sweedler

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Abstract

Matrix-assisted laser desorption-ionization (MALDI) mass spectrometry (MS) is a rapid and sensitive analytical approach that is well suited for obtaining molecular weights of peptides and proteins from complex samples. MALDI-MS can profile the peptides and proteins from single-cell and small tissue samples without the need for extensive sample preparation, except for the cell isolation and matrix application. Strategies for peptide identification and characterization of post-translational modifications are presented. Furthermore, several recent enhancements in MALDI-MS technology, including in situ peptide sequencing as well as the direct spatial mapping of peptides in cells and tissues are discussed.

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Animals Immunohistochemistry Peptides Protein Processing, Post-Translational Sequence Analysis, Protein Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.Review

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L Li, R W Garden, J V Sweedler. 2000. Single-cell MALDI: a new tool for direct peptide profiling.. https://doi.org/10.1016/s0167-7799(00)01427-x

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