PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 10764940

Microcomputer program for automated action potential waveform analysis.

E Soto·E Salceda·R Cruz·A Ortega·R Vega
View original source ↗Export metadata ↓

Abstract

A program for action potential waveform analysis based on a PC compatible computer is described. Single or averaged action potentials are analyzed by obtaining its first derivative and using criteria which allow automatic measurement of several action potential components, including: depolarization rate, repolarization rate, amplitude, duration, resting membrane potential and afterhyperpolarization amplitude and slope. Data can be imported from pClamp (Axon Instruments) and exported to other software such as Excel, Sigmaplot and MatLab for example.

Explore related subjects

Action PotentialsElectronic Data ProcessingElectrophysiologyHumansMicrocomputersSoftwareJournal ArticleResearch Support, Non-U.S. Gov't

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

E Soto, E Salceda, R Cruz, A Ortega, R Vega. 2000. Microcomputer program for automated action potential waveform analysis.. https://doi.org/10.1016/s0169-2607(00)00053-5

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2001

Identification of visual pallial telencephalon in the goldfish, Carassius auratus: a combined cytochrome oxidase and electrophysiological study.

A strategy based upon a comparative decrease in bilateral symmetry of cytochrome oxidase (COX) histochemistry following unilateral eye enucleation was used to identify the primary visual area in the area dorsalis of the telencephalon of the goldfish, Carassius auratus. The lateral zone of area dorsalis (Dl) at about the level of the anterior commissure exhibits such a bilateral difference. A parallel decline in the symmetry COX reactivity was observed in the associated part of the central zone (Dc). Electrophysiological activity using extracellular techniques confirmed the visually-driven activity of neurons in these areas. Lesions confirmed the loci in the lateral zone of area dorsalis, including both its dorsal and ventral parts. Single- and multi-unit recordings exhibited a variety of responses to different light stimuli. Single unit latency measures proved not to be a reliable measure of target areas. Responses habituated to stimuli repeated within 5 s and were only reliably evoked with intervals greater than several seconds.

Action Potentials↗
PubMed2001

Effects of galanin receptor agonists on locus coeruleus neurons.

Galanin exerts an inhibitory effect on locus coeruleus (LC) neurons via a postsynaptic, as yet unidentified galanin receptor. Using an in vitro intracellular recording technique the effect of two galanin receptor agonists on LC neurons was investigated. Bath application of [Sar(1), D-Ala(12)]gal(1-16)-NH(2) (AR-M961), an agonist both at galanin R1 and R2 (GALR1, GALR2) receptors, evoked a reversible membrane hyperpolarization and inhibition of spike discharge in all LC neurons tested (n=42). The action of AR-M961 was blocked by tetraethylammonium chloride. Hyperpolarizing responses induced by AR-M961 were retained in the presence of tetrodotoxin and high Mg(2+)/low Ca(2+) media. The selective GALR2 agonist Gal(2-11)-NH(2) (AR-M1896) only caused inhibition of spike discharge and a slight hyperpolarization in 26 of 34 LC neurons tested, and was on a molar basis much weaker than AR-M961. These results suggest that it mainly is the GALR1 receptor that mediates hyperpolarization of LC neurons.

Action Potentials↗
PubMed2001

Further studies of bulk and orosensory decrement in producing satiation of feeding in Aplysia.

Prior evidence has suggested that meal satiation in the marine mollusk Aplysia is associated with stretch of the crop. The current data, however, suggest that under some conditions, bulk in the crop can be dissociated from the propensity to feed. The crop was hyper-distended 6 h after a satiating meal of rehydrated seaweed; that is, the crop took in water and therefore contained a greater volume than it had contained immediately after satiation. Animals presented with food 6 h after an initial satiating meal consumed a new meal despite the fact that their crop was distended beyond the level at which they had previously terminated feeding. This unexpected result led to additional experiments designed to study possible orosensory decrement during presentation of food. Orosensory input was assessed by recording from the metacerebral cell (MCC) in free-moving animals. The MCC receives excitatory input in response to chemosensory stimulation of the lips, and exhibited a slow decrement during the course of a meal or during repeated lip stimulation without ingestion. Lesions of the cerebro-buccal connectives abolished the long-term MCC response decrement to lip stimulation. This result suggests that the MCC long-term response decrement to lip stimulation is a product of buccal-ganglion feedback and may not reflect sensory decrement of chemosensory pathways. Therefore, satiation may not produce a change in lip sensitivity to chemosensory input. Our data suggest that one important factor that determines satiation is a stretch stimulus of the posterior esophagus/anterior crop. This stretch stimulus may subside over several hours as the crop contents are redistributed or as receptors slowly adapt.

Action Potentials↗