Sensitive single-stage PCR using custom-synthesized internal controls.

K Zimmermann; M Rieger; P Gross; P L Turecek; H P Schwarz

doi:10.2144/00284st05

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PubMed · 10769747

Sensitive single-stage PCR using custom-synthesized internal controls.

K Zimmermann·M Rieger·P Gross·P L Turecek·H P Schwarz

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Abstract

A new approach for an internally controlled PCR was developed using a custom-synthesized oligonucleotide as the internal control. Three different PCR setups demonstrated the usefulness of this approach: (i) the addition of the respective internal control to samples containing ssDNA virus Parvo B19; (ii) the co-extraction of plasma samples and the respective internal control for the detection of the ssDNA virus TTV; and (iii) the addition of the respective internal control to a crude lysate of tail pieces for the genotyping of FVIII knockout mice, demonstrating that this approach is also applicable for dsDNA.

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Animals DNA Primers DNA Virus Infections DNA Viruses DNA, Single-Stranded DNA, Viral Factor VIII Genotype Hepatitis, Viral, Human Humans Mice Mice, Knockout Parvovirus B19, Human Polymerase Chain Reaction Sensitivity and Specificity Journal Article

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K Zimmermann, M Rieger, P Gross, P L Turecek, H P Schwarz. 2000. Sensitive single-stage PCR using custom-synthesized internal controls.. https://doi.org/10.2144/00284st05

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