Single large DNA molecule analysis using fluorescence microscopy.

H Oana; M Ueda

doi:10.1093/nass/42.1.243

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PubMed · 10780470

Single large DNA molecule analysis using fluorescence microscopy.

H Oana·M Ueda

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Abstract

A large DNA analysis method which enable to obtain spatial information of positions of specific sequences along DNA molecule has been developed. Making use of the phenomenon that large DNA molecule is elongated stably under alternative current field in a concentrated linear polymer solution, direct observation of elongated individual lambda DNA molecules with fluorescence probes was carried out using fluorescence microscopy. Then, the spatial positions of the fluorescence spot of the probe on the DNA molecule were determined by image analysis.

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Bacteriophage lambda Base Sequence DNA DNA, Viral Deoxyribonuclease EcoRI Fluorescent Dyes Microscopy, Fluorescence Restriction Mapping Journal Article Research Support, Non-U.S. Gov't

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H Oana, M Ueda. 1999. Single large DNA molecule analysis using fluorescence microscopy.. https://doi.org/10.1093/nass%2F42.1.243

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