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DNA structures recognized by the human UV-DDB protein.

T Mizukoshi·Y Fujiwara·S Iwai
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Abstract

DNA recognition by the human UV-damaged DNA-binding (UV-DDB) protein was characterized. By circular permutation analyses, DNA duplexes containing the (6-4) photoproduct and the abasic site analog were found to be bent at angles of 54 degrees and 57 degrees, respectively, when they formed a complex with this protein. Although kinked NMR structures have been reported, fluorescence resonance energy transfer experiments revealed that these duplexes had no intrinsic bend. These results suggest that the UV-DDB protein binds DNA that can be bent easily at the above angle.

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Base SequenceDNADNA-Binding ProteinsEnergy TransferHumansNuclear Magnetic Resonance, BiomolecularNucleic Acid ConformationOligodeoxyribonucleotidesRestriction MappingSpectrometry, FluorescenceSubstrate SpecificityJournal Article

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T Mizukoshi, Y Fujiwara, S Iwai. 1999. DNA structures recognized by the human UV-DDB protein.. https://doi.org/10.1093/nass%2F42.1.265

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