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PubMed · 10783753

Immunosuppressive activity in buffalo placenta.

S Mehta·Y S Rajput
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Abstract

Immunosuppressive activity in buffalo placenta was evaluated by measuring proliferation of lymphocytes in presence of phytohaemagglutinin (PHA) alone or PHA plus placental proteins. The immunosuppressive activity was dose-dependent over the protein concentration range of 10-50 micrograms/ml. Proteins from both cotyledon and non-cotyledon portions of placenta exhibited immunosuppressive activity. Fractions obtained with 0-40, 40-60 and 60-80% saturated ammonium sulphate exhibited 70, 73 and 75% suppression, respectively. PBS-soluble placental proteins were resolved on G-100 column into three peaks that exhibited 69 (peak 1), 55 (peak 2) and 73% (peak 3) suppression. Exogenously added interleukin-1 (IL-1) failed to reverse the suppression caused by buffalo placental proteins.

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AnimalsBuffaloesFemaleImmune ToleranceIn Vitro TechniquesInterleukin-1Lymphocyte ActivationPlacentaPregnancyPregnancy ProteinsT-LymphocytesJournal Article

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S Mehta, Y S Rajput. 1999. Immunosuppressive activity in buffalo placenta.. https://pubmed.ncbi.nlm.nih.gov/10783753/

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