Screening hydrolysis over two-phases.

M G Cotenescu; J J La Clair

doi:10.1016/s0168-1656(99)00156-x

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PubMed · 10784294

Screening hydrolysis over two-phases.

M G Cotenescu·J J La Clair

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Abstract

A new assay is described that monitors hydrolysis with the concurrent transfer of a solvatochromic dye across an oil-water barrier. Through the appropriate design, this transfer is accompanied by a 10(6) gain in fluorescence. This response can be used to effectively screen hydrolytic activity at high-throughput. Using this method, microunits of alkaline phosphatase, glucosidases, as well as several common proteases can be visually detected within an hour through concentration over a 200:1 volumetric ratio of aqueous to organic phases. Development of a water-solublizing protecting group extends this methodology to screen a wide range of processes that undergo cleavage of a covalent bond.

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Alkaline Phosphatase Endopeptidases Glucosidases Hydrolysis Spectrum Analysis Journal Article

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M G Cotenescu, J J La Clair. 2000-01-07. Screening hydrolysis over two-phases.. https://doi.org/10.1016/s0168-1656(99)00156-x

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