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Partial purification and characterization of a lymphotoxic factor from multiple sclerosis serum.

G White·S Armentrout·S van den Noort
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Abstract

A factor found in the serums of some patients with multiple sclerosis has been shown to have a toxic effect on the normal, mature lymphocyte. This lymphotoxic factor is a protein that is stable to heating at 56 degrees C for 3 hours. It is a nondialyzable material, with recovery of lymphotoxic activity following 30 days of dialysis. Elution of lymphotoxic serum through Sepadex G-100 demonstrates toxic activity closely associated with albumin. Purification by elution through DEAE-cellulose yielded lymphotoxic activity. The lymphotoxic factor was inactivated by standard ammonium sulfate precipitation. Analysis of the effect on distinctive cell types within the total lymphocyte population demonstrated that the T lymphocyte was particularly sensitive.

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Ammonium SulfateAntilymphocyte SerumB-LymphocytesBlood ProteinsChemical PrecipitationChromatography, DEAE-CelluloseChromatography, GelChromatography, Ion ExchangeCytotoxicity Tests, ImmunologicDialysisHot TemperatureHumansMultiple SclerosisT-LymphocytesTime FactorsJournal Article

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G White, S Armentrout, S van den Noort. 1975. Partial purification and characterization of a lymphotoxic factor from multiple sclerosis serum.. https://doi.org/10.1212/wnl.25.1.37

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