Smart and biofunctional streptavidin.

P S Stayton; K E Nelson; T C McDevitt; V Bulmus; T Shimoboji; Z Ding; A S Hoffman

doi:10.1016/s1050-3862(99)00043-1

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PubMed · 10796990

Smart and biofunctional streptavidin.

P S Stayton·K E Nelson·T C McDevitt·V Bulmus·T Shimoboji·Z Ding·A S Hoffman

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Abstract

The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. While it is extremely useful as the native protein, there are many applications where its function can be improved re-engineering the subunits. We review here our efforts to construct streptavidin tetramers that have 'smart' recognition capabilities, and which display functional peptide sequences. These smart and biofunctional streptavidin derivatives can 'talk' to cells, and 'listen' to external signals which control capture and release of biotinylated molecules.

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Affinity Labels Binding Sites Biotechnology Biotin Models, Molecular Protein Conformation Protein Engineering Protein Structure, Quaternary Recombinant Fusion Proteins Streptavidin Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.Review

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P S Stayton, K E Nelson, T C McDevitt, V Bulmus, T Shimoboji, Z Ding, A S Hoffman. 1999-12-31. Smart and biofunctional streptavidin.. https://doi.org/10.1016/s1050-3862(99)00043-1

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